In a groundbreaking initiative, the Padmaja Naidu Himalayan Zoological Park in Darjeeling has launched India’s first wildlife DNA preservation project, commonly referred to as a “frozen zoo.” This pioneering endeavor aims to collect and preserve genetic materials from endangered species native to the Eastern Himalayas, such as the red panda, snow leopard, Tibetan wolf, screw-horned goat, and Himalayan black bear. By safeguarding these DNA samples, the project aspires to bolster conservation efforts and potentially aid in the revival of species facing extinction.
Genesis of the Wildlife DNA Preservation Project
The inception of this “frozen zoo” stems from a collaborative effort between the Darjeeling Zoo and the Centre for Cellular and Molecular Biology (CCMB), operating under the Ministry of Science and Technology. Established in July 2024, the facility has since amassed DNA, cellular, and tissue samples from 60 animals across 23 species, prioritizing those classified as endangered. These samples are meticulously preserved at a temperature of minus 196 degrees Celsius in liquid nitrogen, ensuring their viability for future research and conservation applications.
The decision to launch this initiative was influenced by the increasing threats of habitat loss, climate change, poaching, and inbreeding among endangered species. Scientists believe that by preserving genetic diversity, they can contribute to sustainable conservation programs and mitigate the risk of species extinction.
Objectives and Significance
The primary objective of the wildlife DNA preservation project is to maintain a repository of genetic materials that can be utilized for:
- Genetic Research: Facilitating studies on genetic diversity, adaptation, and disease resistance among endangered species.
- Assisted Reproduction: Employing techniques such as artificial insemination, in-vitro fertilization, and embryo transfer to enhance breeding programs.
- Species Revival: Potentially resurrecting species that have become extinct or are on the brink of extinction through advanced biotechnological methods.
- Improving Conservation Strategies: By understanding the genetic composition of different species, scientists can devise more effective conservation strategies, including selective breeding programs.
- Combatting Inbreeding and Genetic Disorders: Many endangered species suffer from genetic disorders due to inbreeding in small, isolated populations. The DNA bank can help introduce genetic diversity to prevent hereditary diseases.
This initiative is particularly crucial for the Eastern Himalayan region, renowned for its rich biodiversity, housing over 300 mammal species. By preserving genetic materials from wild populations, the project ensures greater genetic diversity, which is vital for the health and resilience of species.
Operational Framework
The success of the wildlife DNA preservation project hinges on a meticulously designed operational framework:
- Sample Collection
Tissue samples are collected from both living and deceased animals. In cases of illness or accidental death, forest officials must act swiftly, ideally within an hour, to preserve the tissue in appropriate solutions for transportation to the laboratory. The collected samples include:
- Blood samples for genetic analysis
- Tissue samples for cryopreservation
- Hair and feather samples for species identification
- Sperm and egg samples for future breeding programs
- Cryopreservation
Collected samples are stored in cryogenic conditions at minus 196 degrees Celsius using liquid nitrogen, maintaining the integrity of the genetic material. This technique ensures that the genetic blueprint of a species remains intact for centuries, allowing future generations to use these samples for scientific advancements.
- Collaboration
The project involves seamless coordination between veterinary professionals, conservationists, and researchers to ensure the ethical and effective collection and utilization of genetic samples. The Darjeeling Zoo works in tandem with international and national scientific bodies to follow global best practices in wildlife conservation.

Comparative Analysis with Global Initiatives
The concept of a “frozen zoo” is not novel globally. The first such facility was established in San Diego in 1972, currently housing over 8,400 samples from more than 800 species. Similar cryobanks exist in the United Kingdom, Germany, and China, serving as critical backups for species threatened by habitat loss and poaching. India’s foray into this domain signifies a significant leap in its wildlife conservation efforts, aligning with global best practices.
One of the most successful global case studies includes the Lazarus Project, which aimed to revive the extinct gastric-brooding frog. Although the species was not successfully revived, the project demonstrated that de-extinction might be feasible with technological advancements.
Challenges and Considerations
While the wildlife DNA preservation project holds immense promise, it also faces several challenges:
- Ethical Concerns
The collection and potential use of genetic materials raise ethical questions regarding animal rights and the implications of de-extinction. Some conservationists argue that focusing on preserving current ecosystems should take precedence over reviving extinct species.
- Technical Limitations
Advanced reproductive technologies required for utilizing preserved DNA are still evolving and may not guarantee success. Even in well-established frozen zoos, only a fraction of stored genetic materials has been successfully used for breeding endangered species.
- Resource Intensiveness
Maintaining cryogenic facilities demands substantial financial and infrastructural resources, posing sustainability concerns. The process requires state-of-the-art equipment, trained personnel, and consistent funding from government and private organizations.
- Legal and Policy Frameworks
Regulatory challenges may arise concerning the use of stored genetic materials, particularly in species revival attempts. Conservation policies need to be updated to address emerging challenges and ethical dilemmas.
Future Prospects
Looking ahead, the Darjeeling Zoo’s initiative could pave the way for similar projects across India, particularly in other biodiversity hotspots. Plans are reportedly underway to establish biobanks in the National Zoological Park in Delhi and Nandankanan Zoo in Odisha. Such expansions would create a robust network for genetic preservation, enhancing the country’s capacity to protect and revive its rich wildlife heritage.
Additionally, researchers are exploring the possibilities of:
- Genome Editing: Technologies like CRISPR could be used to modify genes and help endangered species adapt to changing environmental conditions.
- Cross-Breeding Programs: Utilizing genetic material from similar species to boost populations of critically endangered animals.
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Educational and Awareness Campaigns: Encouraging the general public to support conservation efforts through citizen science initiatives and awareness programs.
Government Support
The Indian government has played a crucial role in supporting the Darjeeling Zoo’s wildlife DNA preservation project through various ministries and agencies. The following government bodies have been instrumental in funding and policy implementation:
- Ministry of Environment, Forest and Climate Change – Website
- Department of Biotechnology, Government of India – Website
- Wildlife Institute of India – Website
- Zoological Survey of India – Website
The launch of the wildlife DNA preservation project at Darjeeling Zoo marks a transformative moment in India’s conservation landscape. By embracing cutting-edge biotechnological approaches, this initiative offers a beacon of hope for endangered species, ensuring that future generations may continue to witness the majestic biodiversity that the Eastern Himalayas have to offer.
With unwavering support from the government, scientific community, and conservationists, the frozen zoo could redefine wildlife conservation in India. As technology advances, this initiative will undoubtedly play a crucial role in protecting the country’s invaluable fauna from the threats of extinction.
Also Read: Rhino Population in West Bengal Surges: 5 Powerful Insights on Conservation Success